primescripttm one step rt pcr kit (TaKaRa)
TaKaRa is a verified supplier 
TaKaRa manufactures this product 
98
Structured Review
TaKaRa
primescripttm one step rt pcr kit
Primescripttm One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pmc13195868-92-16-21
Average 98 stars, based on 10903 article reviews
Primescripttm One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pmc13195868-92-16-21
Average 98 stars, based on 10903 article reviews
primescripttm one step rt pcr kit - by Bioz Stars,
2026-09
98/100 stars
Images
1) Product Images from "Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves"
Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves
Journal: Veterinary Research
doi: 10.1186/s13567-026-01765-3
Figure Legend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
Techniques Used: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control
Related Articles
Reverse Transcription Polymerase Chain Reaction:Article Title: Therapeutic base editing alleviates restrictive cardiomyopathy Article Snippet: Uniclone One Step Seamless Cloning Kit , Genesand , SC612. .. Article Title: Intelligent nanoliposome ameliorate chronic intermittent hypoxia-mediated neuronal injury via a dual regulation microglial inflammation strategy Article Snippet: Levels of IL-1β and TNF-α in supernatants were measured by ELISA kits (BioLegend) following the manufacturers’ instructions. .. Total RNA was isolated by Trizol reagent (10296028, Invitrogen), and complementary DNA was generated using the Takara RNA PCR kit (RR420A, Takara). qRT‐PCR was performed with Article Title: Low-Fishmeal Dietary Supplementation with Crayfish By-Product Protein Hydrolysate Affects Growth Performance, Appetite-Related Metabolic Signaling and Intestinal Microbiota of Pacific White Shrimp ( Litopenaeus vannamei ) Article Snippet: .. Reverse transcription was performed to generate first-strand cDNA using the Article Title: Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. Article Snippet: Lentiviral overexpression vectors (Ubc-3FLAG-CBh-gcGFP-IRES-puromycin; PLS1-WT and PLS1-ΔExon8) were produced by GeneChem and transduced into HEK293T and HEI-OC1 (Pls1-KO) cells according to the manufacturer’s protocol. .. Puromycin selection was performed at 72 h post-transduction to establish stable cell lines. qRT-PCR Total RNA was extracted using an RNA extraction kit (Promega, Shanghai, China), according to the manufacturer’s protocol. cDNA synthesis was performed using the Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the Article Title: Inflammatory macrophage-derived plasminogen activator inhibitor-1 exacerbates inflammation through efferocytosis inhibition. Article Snippet: .. The RNA (0.5 μg) was reverse-transcribed to cDNA using the Article Title: Multi-strategy engineering of Saccharomyces cerevisiae for enhanced β-ionone biosynthesis Article Snippet: β-Ionone is a natural volatile compound known for its distinctive violet and woody aroma, with broad application potential in the flavor, fragrance, and pharmaceutical industries.. To meet the growing market demand for natural products and overcome the limitations of chemical synthesis and plant extraction, microbial cell factories have emerged as a sustainable and environmentally friendly alternative.. In this study, a positive correlation was established between β-ionone production and the accumulation of its precursor, β-carotene. Isolation:Article Title: Intelligent nanoliposome ameliorate chronic intermittent hypoxia-mediated neuronal injury via a dual regulation microglial inflammation strategy Article Snippet: Levels of IL-1β and TNF-α in supernatants were measured by ELISA kits (BioLegend) following the manufacturers’ instructions. .. Total RNA was isolated by Trizol reagent (10296028, Invitrogen), and complementary DNA was generated using the Takara RNA PCR kit (RR420A, Takara). qRT‐PCR was performed with Generated:Article Title: Intelligent nanoliposome ameliorate chronic intermittent hypoxia-mediated neuronal injury via a dual regulation microglial inflammation strategy Article Snippet: Levels of IL-1β and TNF-α in supernatants were measured by ELISA kits (BioLegend) following the manufacturers’ instructions. .. Total RNA was isolated by Trizol reagent (10296028, Invitrogen), and complementary DNA was generated using the Takara RNA PCR kit (RR420A, Takara). qRT‐PCR was performed with Polymerase Chain Reaction:Article Title: Intelligent nanoliposome ameliorate chronic intermittent hypoxia-mediated neuronal injury via a dual regulation microglial inflammation strategy Article Snippet: Levels of IL-1β and TNF-α in supernatants were measured by ELISA kits (BioLegend) following the manufacturers’ instructions. .. Total RNA was isolated by Trizol reagent (10296028, Invitrogen), and complementary DNA was generated using the Takara RNA PCR kit (RR420A, Takara). qRT‐PCR was performed with Real-time Polymerase Chain Reaction:Article Title: Intelligent nanoliposome ameliorate chronic intermittent hypoxia-mediated neuronal injury via a dual regulation microglial inflammation strategy Article Snippet: Levels of IL-1β and TNF-α in supernatants were measured by ELISA kits (BioLegend) following the manufacturers’ instructions. .. Total RNA was isolated by Trizol reagent (10296028, Invitrogen), and complementary DNA was generated using the Takara RNA PCR kit (RR420A, Takara). qRT‐PCR was performed with Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the Article Title: Inflammatory macrophage-derived plasminogen activator inhibitor-1 exacerbates inflammation through efferocytosis inhibition. Article Snippet: .. The RNA (0.5 μg) was reverse-transcribed to cDNA using the Article Title: Multi-strategy engineering of Saccharomyces cerevisiae for enhanced β-ionone biosynthesis Article Snippet: β-Ionone is a natural volatile compound known for its distinctive violet and woody aroma, with broad application potential in the flavor, fragrance, and pharmaceutical industries.. To meet the growing market demand for natural products and overcome the limitations of chemical synthesis and plant extraction, microbial cell factories have emerged as a sustainable and environmentally friendly alternative.. In this study, a positive correlation was established between β-ionone production and the accumulation of its precursor, β-carotene. Reverse Transcription:Article Title: Low-Fishmeal Dietary Supplementation with Crayfish By-Product Protein Hydrolysate Affects Growth Performance, Appetite-Related Metabolic Signaling and Intestinal Microbiota of Pacific White Shrimp ( Litopenaeus vannamei ) Article Snippet: .. Reverse transcription was performed to generate first-strand cDNA using the Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the Article Title: Inflammatory macrophage-derived plasminogen activator inhibitor-1 exacerbates inflammation through efferocytosis inhibition. Article Snippet: .. The RNA (0.5 μg) was reverse-transcribed to cDNA using the Selection:Article Title: Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. Article Snippet: Lentiviral overexpression vectors (Ubc-3FLAG-CBh-gcGFP-IRES-puromycin; PLS1-WT and PLS1-ΔExon8) were produced by GeneChem and transduced into HEK293T and HEI-OC1 (Pls1-KO) cells according to the manufacturer’s protocol. .. Puromycin selection was performed at 72 h post-transduction to establish stable cell lines. qRT-PCR Total RNA was extracted using an RNA extraction kit (Promega, Shanghai, China), according to the manufacturer’s protocol. cDNA synthesis was performed using the Stable Transfection:Article Title: Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. Article Snippet: Lentiviral overexpression vectors (Ubc-3FLAG-CBh-gcGFP-IRES-puromycin; PLS1-WT and PLS1-ΔExon8) were produced by GeneChem and transduced into HEK293T and HEI-OC1 (Pls1-KO) cells according to the manufacturer’s protocol. .. Puromycin selection was performed at 72 h post-transduction to establish stable cell lines. qRT-PCR Total RNA was extracted using an RNA extraction kit (Promega, Shanghai, China), according to the manufacturer’s protocol. cDNA synthesis was performed using the Quantitative RT-PCR:Article Title: Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. Article Snippet: Lentiviral overexpression vectors (Ubc-3FLAG-CBh-gcGFP-IRES-puromycin; PLS1-WT and PLS1-ΔExon8) were produced by GeneChem and transduced into HEK293T and HEI-OC1 (Pls1-KO) cells according to the manufacturer’s protocol. .. Puromycin selection was performed at 72 h post-transduction to establish stable cell lines. qRT-PCR Total RNA was extracted using an RNA extraction kit (Promega, Shanghai, China), according to the manufacturer’s protocol. cDNA synthesis was performed using the Article Title: Multi-strategy engineering of Saccharomyces cerevisiae for enhanced β-ionone biosynthesis Article Snippet: β-Ionone is a natural volatile compound known for its distinctive violet and woody aroma, with broad application potential in the flavor, fragrance, and pharmaceutical industries.. To meet the growing market demand for natural products and overcome the limitations of chemical synthesis and plant extraction, microbial cell factories have emerged as a sustainable and environmentally friendly alternative.. In this study, a positive correlation was established between β-ionone production and the accumulation of its precursor, β-carotene. RNA Extraction:Article Title: Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. Article Snippet: Lentiviral overexpression vectors (Ubc-3FLAG-CBh-gcGFP-IRES-puromycin; PLS1-WT and PLS1-ΔExon8) were produced by GeneChem and transduced into HEK293T and HEI-OC1 (Pls1-KO) cells according to the manufacturer’s protocol. .. Puromycin selection was performed at 72 h post-transduction to establish stable cell lines. qRT-PCR Total RNA was extracted using an RNA extraction kit (Promega, Shanghai, China), according to the manufacturer’s protocol. cDNA synthesis was performed using the cDNA Synthesis:Article Title: Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model. Article Snippet: Lentiviral overexpression vectors (Ubc-3FLAG-CBh-gcGFP-IRES-puromycin; PLS1-WT and PLS1-ΔExon8) were produced by GeneChem and transduced into HEK293T and HEI-OC1 (Pls1-KO) cells according to the manufacturer’s protocol. .. Puromycin selection was performed at 72 h post-transduction to establish stable cell lines. qRT-PCR Total RNA was extracted using an RNA extraction kit (Promega, Shanghai, China), according to the manufacturer’s protocol. cDNA synthesis was performed using the Article Title: DHT-Induced lncRNA AC092718.4 Promotes Prostate Cancer Cell Proliferation via ceRNA Mechanism Article Snippet: Total RNA was isolated using the RNeasy®Plus mini Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer’s protocol. .. Reverse transcription of total RNA was carried out using the Article Title: Red light pretreatment promotes esters biosynthesis by activating the CmBBX7/14-CmLOX18-CmAAT1 module in postharvest oriental melon at low temperature Article Snippet: Combining light quality regulation with postharvest low-temperature storage holds promise for mitigating postharvest aroma loss in fruits.. However, the molecular mechanism by which light quality modulates postharvest aroma biosynthesis remains unclear.. In this study, ‘Bali Xiang’ oriental melon fruit at the early stage of ethylene release were selected as experimental material and subjected to red light (RLT) or blue light (BLT) pretreatment prior to low-temperature storage. Gene Expression:Article Title: Inflammatory macrophage-derived plasminogen activator inhibitor-1 exacerbates inflammation through efferocytosis inhibition. Article Snippet: .. The RNA (0.5 μg) was reverse-transcribed to cDNA using the |